San Diego – A new study reveals that a protein long known to play a role in communication between cells in the brain is also capable of obliterating cells if left unchecked because of its penchant for twisting and puncturing the cell membranes.
The protein — known as complexin — if left alone is so toxic it can shred cells. Yet, in the brain, a suite of controls makes sure the protein plays nice and helps neurons communicate by aiding in the release of neurotransmitters.
The findings are published Feb. 7 in Nature Structural and Molecular Biology. "We argue that’s the most interesting membrane fusion event in our bodies, because it’s the one that underlies this conversation. It controls remembering and forgetting. It is everything," says Ed Chapman, professor of neuroscience at the University of Wisconsin School of Medicine and Public Health. "Yet to this day, nobody knows just how the proteins involved in this process really work."
In trying to better understand these proteins Chapman's lab and their collaborators discovered the surprising power of complexin. They found that it dramatically bends and reorders membranes. Live videos show the protein pinching off small bubbles of membrane while simultaneously poking holes in them. Ultrahigh-resolution 3D images produced by the laboratories of Dorit Hanein and Niels Volkmann at the Scintillon Institute in San Diego also revealed, directly through Cryo-EM imaging, that complexin induces the formation of twisting curlicues of broken-apart membranes.Read more