CDW Tutorials 2019
CDW Tutorials: Single Extracellular Vesicle (EV) Analytics
THURSDAY, NOVEMBER 14th, 2019
Scintillon Institute
Organized in cooperation with the ISEV-ISAC-ISTH EV Flow Cytometry Working Group, the University of California, San Diego and Scintillon Institute, this year's CDW Tutorials will focus on single EV analytics. The Tutorials will include lectures on EV biogenesis, transport and function, EV isolation and enrichment, and EV measurement, with a focus on MIFlowCyt-EV, the recently developed Guidelines for reporting EV flow cytometry methods and results. The program will consist of morning lectures and afternoon wet labs that illustrate essential aspects of experimental design and measurement calibration.
Due to the small group format of this year's Tutorials, space is limited to 24 attendees. If you are interested in attending, please send an email with a brief description of your interest and experience with extracellular vesicles and/or flow cytometry to: [email protected] with "Tutorial Application" in the subject line by October 31, 2019.
Preliminary Agenda
|
Morning |
Lecture |
Speaker |
|
8:30- |
Welcome and Overview |
John Nolan |
|
8:45- |
EV Research and Methods |
Ken Witwer |
|
9:10- |
Single EV measurements: Instruments and Assays |
Joshua Welsh |
|
9:30- |
Single EV Flow Cytometry: Reporting Guidelines |
Joanne Lannigan |
|
10:00- |
Break and sponsor presentations |
|
|
11:00- |
EV Light Scatter by Flow Cytometry |
Joshua Welsh |
|
11:20- |
EV Fluorescence by Flow Cytometry |
Joanne Lannigan |
|
11:40- |
Introduction to the Lab Modules |
John Nolan |
|
12:00 |
Lunch |
|
|
Afternoon |
Wet Labs |
|
|
1:00- |
Module 1: Single EV Counting and Sizing |
Ngoc Do, JB Vasquez and Patrick Nolan |
|
|
Module 2: Experimental Design for Single EV Analysis |
Joanne Lannigan |
|
|
Module 3: Single EV Light Scatter Flow Cytometry |
Joshua Welsh and Vera Tang |
|
|
Module 4: Single EV Fluorescence Flow Cytometry |
John Nolan and Erika Duggan |
|
5:30 |
Tutorial Wrap up |
All |
Scintillon Institute
6868 Nancy Ridge Dr
San Diego, CA 92121
CDW 2019 Sponsors:
 |
 |
 |
 |
 |
 |
 |
PAST TUTORIAL SCHEDULE: 2017