Cytometry Development Tutorials on

Single EV Analytics

THURSDAY, NOVEMBER 14, 2019

SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE BLDG 12

Produced in cooperation with the ISEV-ISAC-ISTH EV FC Working Group

Faculty: Joanne Lannigan, John Nolan, Joshua Welsh, Ken Wittwer, TBA

Agenda (draft)

Morning – Lectures

The morning session will include lecture-based overviews of EV research and methods, minimum Information and reporting guidelines, and single vesicle measurement technologies. Tentative Agenda:

Overview of EVs, EV research, and EV methods
Biofluid fractionation and EV enrichment
Single EV measurement: Instruments and assays
Single EV FC: Guidelines and examples

Afternoon – Lab sessions

The afternoon sessions will feature hands-on lab modules to demonstrate essential aspects of single EV measurements, with an emphasis on multiparameter analysis by flow cytometry and the recently developed guidelines for reporting EV FC analysis methods and results. Tentative Agenda:

Lab 1 – Nanoparticle counting and sizing: RPS and NTA

Lab 2 – EV detection and sizing by FC: Light scatter

Lab 3 – EV counting and sizing by FC: Fluorescence

Lab 4 – EV immunofluorescence by FC