CDW Tutorials 2019

CDW Tutorials: Single Extracellular Vesicle (EV) Analytics

THURSDAY, NOVEMBER 14th, 2019

Sanford Consortium for Regenerative Medicine

Organized in cooperation with the ISEV-ISAC-ISTH EV Flow Cytometry Working Group and hosted by the University of California, San Diego, this year's CDW Tutorials will focus on single EV analytics. The Tutorials will include lectures on EV biogenesis, transport and function, EV isolation and enrichment, and EV measurement, with a focus on the recently developed Guidelines for reporting EV flow cytometry methods and results. The program will consist of morning lectures and afternoon wet labs that illustrate essential aspects of experimental design and measurement calibration.

Due to the small group format of this year's Tutorials, space is limited to 24 attendees. If you are interested in attending, please send an email with a brief description of your interest and experience with extracellular vesicles and/or flow cytometry to: CDW@scintillon.org with "Tutorial Application" in the subject line by October 31, 2019.

 

Preliminary Agenda

Morning

Lecture

Speaker

8:30-

Overview of EVs, EV research, and EV methods

Ken Witwer

8:50-

Biofluid fractionation and EV enrichment

John Nolan

9:10-

Single EV measurements: Instruments and assays

Joshua Welsh

9:30-

Single EV Flow Cytometry: Guidelines and examples

Joanne Lannigan

10:00-

Break and sponsor presentations

 

11:00-

EV light scatter by flow cytometry

Joshua Welsh

11:20-

EV fluorescence by flow cytometry

John Nolan

11:40-

Introduction to the wet lab modules

Joanne Lannigan

12:00

Lunch

 

Afternoon

Wet Labs

 

1:00-

Module 1: Single EV counting and sizing

TBA

 

Module 2: Experimental design for single EV analysis

Joanne Lannigan

 

Module 3: Single EV light scatter flow cytometry

Joshua Welsh and Vera Tang

 

Module 4: Single EV fluorescence flow cytometry

John Nolan

5:30

Wet Lab Wrap up

All

 

CDW Sponsorship

CDW Housing

CDW 2019 Home

CDW 2019 Sponsors:

CDW-2018.png

PAST TUTORIAL SCHEDULE: 2017

 


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