CDW Tutorials 2019

CDW Tutorials: Single Extracellular Vesicle (EV) Analytics

THURSDAY, NOVEMBER 14th, 2019

Scintillon Institute

Organized in cooperation with the ISEV-ISAC-ISTH EV Flow Cytometry Working Group, the University of California, San Diego and Scintillon Institute, this year's CDW Tutorials will focus on single EV analytics. The Tutorials will include lectures on EV biogenesis, transport and function, EV isolation and enrichment, and EV measurement, with a focus on MIFlowCyt-EV, the recently developed Guidelines for reporting EV flow cytometry methods and results. The program will consist of morning lectures and afternoon wet labs that illustrate essential aspects of experimental design and measurement calibration.

Due to the small group format of this year's Tutorials, space is limited to 24 attendees. If you are interested in attending, please send an email with a brief description of your interest and experience with extracellular vesicles and/or flow cytometry to: CDW@scintillon.org with "Tutorial Application" in the subject line by October 31, 2019.

 

Preliminary Agenda

Morning

Lecture

Speaker

8:30-

Welcome and Overview

John Nolan

8:45-

EV Research and Methods

Ken Witwer

9:10-

Single EV measurements: Instruments and Assays

Joshua Welsh

9:30-

Single EV Flow Cytometry: Reporting Guidelines

Joanne Lannigan

10:00-

Break and sponsor presentations

 

11:00-

EV Light Scatter by Flow Cytometry

Joshua Welsh

11:20-

EV Fluorescence by Flow Cytometry

Joanne Lannigan

11:40-

Introduction to the Lab Modules

John Nolan

12:00

Lunch

 

Afternoon

Wet Labs

 

1:00-

Module 1: Single EV Counting and Sizing

Ngoc Do, JB Vasquez and Patrick Nolan

 

Module 2: Experimental Design for Single EV Analysis

Joanne Lannigan

 

Module 3: Single EV Light Scatter Flow Cytometry

Joshua Welsh and Vera Tang

 

Module 4: Single EV Fluorescence Flow Cytometry

John Nolan and Erika Duggan

5:30

Tutorial Wrap up

All

Tutorials Location

Scintillon Institute

6868 Nancy Ridge Dr

San Diego, CA 92121

 

CDW Sponsorship

CDW Housing

CDW 2019 Home

CDW 2019 Sponsors:

PAST TUTORIAL SCHEDULE: 2017

 


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